A Crucial Tool for AAV Manufacturing: Mass Photometry for Downstream Processing

In the burgeoning field of gene therapies utilizing adeno-associated virus (AAV) vectors, the need for advanced analytical methods has reached an unprecedented high. Process analytical technologies (PAT) play a vital role in ensuring the production of high-quality AAV products. An essential component of PAT is the ability to rapidly and accurately characterize samples based on critical quality attributes (CQAs) such as sample purity.

Enter mass photometry, a revolutionary analytical technology for downstream processing in AAV manufacturing. This state-of-the-art technology offers swift results and consistent measurement of empty-full-partially filled AAV ratios. Its minimal sample requirements and serotype-agnostic nature make it ideally suited for various stages of AAV downstream processing workflows.

In a recent webinar, industry experts Racha Majed, PhD, Technical Sales Specialist at Refeyn, and Quentin Bazot, PhD, Head of Innovation and Process Development at ABL, an Institut Mérieux Company, demonstrated the invaluable utility of mass photometry in characterizing AAV samples. The webinar aimed to provide crucial insights for downstream process development, featuring a compelling case study by ABL that illustrated the practical application of mass photometry in their analytical workflows. The case study highlighted how mass photometry facilitates the rapid and reliable determination of empty-full AAV capsid ratios and partials, thereby accelerating the optimization of the polishing step. It also included a comparison of mass photometry with other methods for measuring empty-full AAV capsid ratios, such as qPCR/ELISA.

Furthermore, the webinar illuminated the broader applications of mass photometry throughout the AAV manufacturing processes. Participants were also provided with insights into a software solution tailored to meet all requirements for GMP-regulated environments.

If you are eager to delve deeper into the realm of mass photometry and its pivotal role in AAV manufacturing, you can access the recorded webcast by simply completing the form below. Do not miss this opportunity to stay abreast of cutting-edge analytical technologies in gene therapy manufacturing.